Combinatorial Control of Transgene Expression by Hypoxia-Responsive Promoter and MicroRNA Regulation for Neural Stem Cell-Based Cancer Therapy
Figure 5
In vivo transgene expression in MCF-7 tumor-bearing mice. (a) Schematic representation of the in vivo expression assay. MCF-7 cells are inoculated into the right mammary fat pad of the mouse and sham injections are given on the contralateral side. After the tumors develop, NSC vectors are inoculated into the tumor sites and sham injection sites, respectively. 24 h after NSC inoculation, luciferase reporter gene expression levels are monitored by live animal imaging. (b) Bioluminescent image showing luciferase reporter gene expression in the tumor-bearing mice. Red circles indicate the inoculation sites of NSC vectors. (c) Average quantitative transgene expression levels in the tumor sites and sham injection sites. Error bars: s.d. , .
