The Low Chamber Pancreatic Cancer Cells Had Stem-Like Characteristics in Modified Transwell System: Is It a Novel Method to Identify and Enrich Cancer Stem-Like Cells?

Figure 6

The lower chamber cells displayed high level of self-renewal related proteins. The expression levels of SHH, Bmi-1, and β-catenin in the lower chamber cells, the bulk Panc-1 cells, and the upper chamber cells were determined by Western-blot analysis. Densitometry analysis revealed the differences between the lower chamber cells, bulk Panc-1 cells, and the upper chamber cells. Data were normalized to β-actin levels. Experiments were repeated three times with similar data. L represents the lower chamber cells, C represents the bulk pancreatic cells, U represents the upper chamber cells (*𝑃<0.05, **𝑃<0.01, and ***𝑃<0.001).