Research Article
Tailored PVA/ECM Scaffolds for Cartilage Regeneration
Table 1
Primers for RT-PCR.
| | Gene | Forward primer 5′→3′
Reverse primer 3′→5′ | GenBank
accession | Base pair
(bp) |
| Collagen, type II, alpha 1
(COL2A1) | F: CCGGGCAGAGGGCAATAGCAGGTT
R: CAATGATGGGGAGGCGTGAG | NM_001844.4 | 127 | Collagen, type IX, alpha 3
(COL9A3) | F: AATCAGGCTCTCGAAGCTCATAAAA
R: CCTGCCACACCCCCGCTCCTTCAT | NM_001853.3 | 99 | Collagen, type X, alpha 1
(COL10A1) | F: GAACTCCCAGCACGCAGAATCC
R: GTGTTGGGTAGTGGGCCTTTTATG | NM_000493.3 | 144 | Cartilage
oligomeric
matrix protein
(COMP) | F: CCGGAGGGTGACGCGCAGATTGA
R: TGCCCTCGAAGTCCACGCCATTGAA | NM_000095.2 | 132 | Aggrecan
(ACAN) | F: GGCTGCTGTCCCCGTAGAAGA
R: GGGAGGCCAAGTAGGAAGGAT | NM_001135.3 | 162 | Transcription
factor SOX9
(SOX9) | F: CTGGGCAAGCTCTGGAGA
R: ATGTGCGTCTGCTCCGTG | NM_000346.3 | 178 | Hyaluronan
synthase 1
(HAS1) | F: CAGACCCACTGCGATGAGAC
R: CCACCAGGTGCGCTGAAA | NM_001523.2 | 217 | Hypoxanthine phosphoribosyltransferase 1
(HPRT1) | F: ATGGACAGGACTGAACGTCTTGCT
R: TTGAGCACACAGAGGGCTACAATG | NM_000194.2 | 79 |
|
|
