In Vitro Screening for β-Hydroxy-β-methylglutaryl-CoA Reductase Inhibitory and Antioxidant Activity of Sequentially Extracted Fractions of Ficus palmata Forsk

Figure 2

(a) DPPH free radical scavenging activity of different FPL extracts. Each value in the figure is represented as mean ± SD (𝑛=3). Nonsignificant (ns), significantly different ^∗𝑃<0.05, ^∗∗𝑃<0.01 versus 0 μg/mL. (b) DPPH free radical scavenging activity of different FPB extracts. Each value in the figure is represented as mean ± SD (𝑛=3). Nonsignificant (ns), significantly different ^∗𝑃<0.05, ^∗∗𝑃<0.01 versus 0 μg/mL. (c) Ferric reducing antioxidant power of different plant extracts. Each value in the figure is represented as mean ± SD (𝑛=3). (d) Percent inhibition of superoxide free radicals by different FPL extracts. Each value in the figure is represented as mean ± SD (𝑛=3). FPLH, FPLD, and FPLE are significantly different; 𝑃<0.05 compared to control (0 μg/mL); FPLM, FPLA, and ascorbic acid are significantly different; 𝑃<0.01 versus 0 μg/mL. (e) Percent inhibition of superoxide free radicals by different FPB extracts. Each value in the figure is represented as mean ± SD (𝑛=3). FPBH, FPBD, and FPBE are significantly different; 𝑃<0.05 compared to control (0 μg/mL); FPBM, FPBA, and ascorbic acid are significantly different; 𝑃<0.01 versus 0 μg/mL. (f) Percent inhibition of lipid peroxidation by FPL extracts. Each value in the figure is represented as mean ± SD (𝑛=3). FPLM, FPLA, and quercetin are significantly different; 𝑃<0.05 compared to control (0 μg/mL); FPLH, FPLD, and FPLE are significantly different; 𝑃<0.01 versus 0 μg/mL. (g) Percent inhibition of lipid peroxidation by FPB extracts. Each value in the figure is represented as mean ± SD (𝑛=3). FPBH and FPBE are significantly different; 𝑃<0.05 compared to control (0 μg/mL); FPBM, FPBA, FPBD, and quercetin are significantly different; 𝑃<0.01 versus 0 μg/mL. (h) Effects of various Ficus virens extracts on supercoiled pUC18 plasmid DNA (100 ng) damage caused by OH^• radicals. L1: pUC18 DNA + PBS (20 μL), L2: pUC18 DNA + Fenton’s reagent (10 μL) + PBS (10 μL), Lanes 3–5: pUC18 DNA + Fenton’s reagent (10 μL) + FPBA (50, 100, 250 μg/10 μL), Lanes 6–8: pUC18 DNA + Fenton’s reagent (10 μL) + FPLM (50, 100, 250 μg/10 μL), and Lane 9: pUC18 DNA + Fenton’s reagent (10 μL) + mannitol (250 μg/10 μL).

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