Minocycline Enhances the Effectiveness of Nociceptin/Orphanin FQ during Neuropathic Pain
Figure 2
Repeated minocycline administration influenced the N/OFQ system parallel to microglia regulation in the spinal cord level under the neuropathic pain. Seven days after CCI in the ipsilateral dorsal spinal cord, minocycline-treatment diminished the level of NOP (b) and IBA-1 (c) proteins levels that were upregulated by nerve injury. The NOP mRNA level was unchanged by nerve injury and minocycline treatment (a). The qRT-PCR and Western blot data are presented as the mean ± SEM and represent the normalized averages derived from analyses of 4–8 samples for each group. Intergroup differences were analyzed using ANOVA followed by Bonferroni’s multiple comparison test. and indicate significant differences compared with naïve rats. and indicate significant differences comp ared with the CCI-treated group. V: vehicle, MC: minocycline.
