Suitability of IS6110-RFLP and MIRU-VNTR for Differentiating Spoligotyped Drug-Resistant Mycobacterium tuberculosis Clinical Isolates from Sichuan in China
Table 3
Allelic diversity of the 24 MIRU-VNTRs loci in 274 drug-resistant M. tuberculosis isolates from Sichuan, China.
Schemes of VNTR locus
VNTR locus
VNTR alias
No. of alleles
Range of repeats
Allelic diversity (HGI) for
Beijing isolates ()
Non-Beijing isolates ()
All isolates ()
Discriminatory loci
424
Mtub04
6
0–4, 6
0.722
0.705
0.745
577
ETR-C
5
2–6
0.111
0.159
0.126
580
MIRU4
7
1–6, 8
0.185
0.65
0.367
802
MIRU40
5
1–5
0.558
0.727
0.643
960
MIRU10
6
0–5
0.589
0.596
0.627
1644
MIRU16
6
0–5
0.664
0.689
0.673
1955
Mtub21
9
1–9
0.706
0.73
0.803
2163b
QUB-11b
15
0–10, 19–22
0.783
0.852
0.829
2165
ETR-A
5
1–5
0.262
0.678
0.502
2401
Mtub30
5
2–6
0.140
0.265
0.438
2996
MIRU26
10
1–10
0.727
0.724
0.79
3192
MIRU31
6
1–6
0.586
0.697
0.708
3690
Mtub39
6
1–6
0.626
0.792
0.708
4052
QUB-26
11
1–11
0.759
0.828
0.782
4156
QUB-4156
7
0–7
0.605
0.479
0.571
Additional loci
154
MIRU2
3
1–3
0
0.07
0.022
2059
MIRU20
2
1-2
0.031
0.069
0.043
2347
Mtub29
4
2–5
0.052
0.093
0.064
2461
ETR-B
3
1–3
0.031
0.488
0.236
2531
MIRU23
7
1, 3–8
0.176
0.199
0.183
2687
MIRU24
1
1
0
0
0
3007
MIRU27
5
0–4
0.599
0.662
0.624
3171
Mtub34
4
1–4
0.120
0.137
0.125
4348
MIRU39
6
1–6
0.734
0.696
0.751
In bold: the HGI > 0.6. The 12-loci-VNTR scheme was developed based on the highly discriminatory loci (HGI > 0.6) for all isolates. Both discriminatory and additional loci were from Supply et al. [31].
