Hepatic Stellate Cell Coculture Enables Sorafenib Resistance in Huh7 Cells through HGF/c-Met/Akt and Jak2/Stat3 Pathways
Figure 3
Exogenous HGF could also trigger sorafenib resistance in Huh7. (a) HGF of indicated concentration was administrated 2 h before sorafenib administration. HGF of 20 ng/mL rescued Huh7 from cell death. (b) DAPI staining was adopted for the manifestation of cell death. HGF of 20 ng/mL decreased sorafenib-induced cell death. (c) Crizotinib reversed HGF-induced cell survival. Crizotinib activated the cleavage of PARP and caspase-9 ( HGF 10 ng/mL versus control; sora: sorafenib).
