Heparin and Liver Heparan Sulfate Can Rescue Hepatoma Cells from Topotecan Action
Figure 2
Differences of topoisomerase I activity in liver samples (a) and in hepatoma cells (b) (representative image of three independent experiments). Topoisomerase I activities in liver samples (a) and hepatoma cell lines (b) of 250 and 500 ng nuclear extract from peritumoral liver (a 1, a 2), hepatocellular carcinoma (a 3, a 4), HepG2 (b 1, b 2), and Hep3B cells (b 3, b 4). The activities of the specimens are related to the amounts of topoisomerase I protein in the cell nuclear extracts, as it is demonstrated on a western blot (c). Lane 1: Hep3B, lanes 2 and 3: HepG2, lanes 4 and 6: hepatocellular carcinomas, and lanes 5 and 7: peritumoral liver tissues. In addition to the 120 kDa band of the whole protein, the antibody reacts with more degradation products of the enzyme, including the 67 kDa catalytic fragment. A: plasmid control without cell nuclear extract. R: relaxed, S: supercoiled plasmid.
