Heparin and Liver Heparan Sulfate Can Rescue Hepatoma Cells from Topotecan Action
Figure 4
Inhibitory effect of heparin, normal and peritumoral liver heparane sulfate (HS), and hepatocellular carcinoma heparane sulfate on the TpT induced topo I cleavage reaction (representative image of three independent experiments). The origin of nuclear extracts: (a): hepatocellular carcinoma; (b): HepG2 cells; (c): Hep3B cells. The reaction mixture contained pBR322 plasmid DNA, nuclear extracts as described, 100 μM topotecan in all samples as well as in the positive controls. In negative control topotecan was not added. Types and concentrations of GAGs are as follows: (a1): heparin 1 μM; (a, b, c 2): heparin 2 μM; (a3): normal liver HS 1 μM; (a, b, c 4): normal liver HS 2 μM; (a5): normal liver HS 3 μM; (a6): peritumoral HS 1 μM; (a, b, c 7): peritumoral HS 2 μM; (a8): hepatocellular carcinoma HS 1 μM; (a, b, c 9): hepatocellular carcinoma HS 2 μM.
