Cardiac Phosphoproteomics during Remote Ischemic Preconditioning: A Role for the Sarcomeric Z-Disk Proteins

Figure 3

Phosphopeptide identification and quantification for phospho-myozenin-2 from RIPC and control samples using Tandem Mass Tagging. P-myozenin-2 peptide phosphorylated at threonine 107 is shown in panel (a). The mass peaks generated by different mass tags (reporter ions) for different samples are magnified and shown in bar chart form in panel (a) for both RIPC (numbers: 126, 127, and 128) and control (129, 130, and 131) samples. The (MS/MS) spectra for the peptide used for protein identification and the reporter ions (boxed) are shown in (b). The samples used in these measurements were taken from Series 1.