Cardiac Phosphoproteomics during Remote Ischemic Preconditioning: A Role for the Sarcomeric Z-Disk Proteins

Table 3

RIPC-induced changes in phosphorylation of myozenin-2 (protein accession: Q9JJW5) as per phosphorylation sites (amino acids shown in small bold and underlined letters). Phosphorylated myozenin-2 was measured using TMT isobaric mass tagging. Data shown are for series 1 (3 control and 3 RIPC). Fold change was calculated by taking average values for control and for RIPC.

Amino acid sequence of fragments	Modified residue(s)	Fold change	𝑃 value	𝑃 value normalised to p-GAPDH
vDGSNLEGGSQQGPStPPNTPDPR	T107	1.67	0.021*	0.043*
vDGSNLEGGSQQGPsTPPNTPDPR	S106	1.55	0.007*	0.025*
vDGSNLEGGsQQGPSTPPNTPDPR	S101	1.34	0.017*	0.078
vDGSNLEGGsQQGPsTPPNTPDPR	S101/S106	1.15	0.290	0.169
vDGSNLEGGsQQGPStPPNTPDPR	S101/T107	1.09	0.665	0.637
vDGSNLEGGSQQGPsTPPNtPDPR	S106/T111	1.15	0.290	0.169
vDGSNLEGGSQQGPStPPNtPDPR	T107/T111	1.09	0.665	0.637

𝑃 value calculated using unpaired 𝑡-test (^∗𝑃<0.05 considered as significant).