Knockdown of AKT3 (PKBγ) and PI3KCA Suppresses Cell Viability and Proliferation and Induces the Apoptosis of Glioblastoma Multiforme T98G Cells
Figure 5
(a) DNA content frequency histograms representing cells from untransfected cultures and from cultures that had been transfected with AKT3, PI3KCA, and AKT3 + PI3KCA siRNAs. (b) The cell cycle distribution of the T98G cell culture that had been transfected with AKT3, PI3KCA, and AKT3 + PI3KCA siRNAs, respectively, compared to the untransfected control cells. Cells were fixed in 70% ethanol and stained with PI. Fluorescence of the PI-stained cells was measured using a FACS AriaII cytometer (Becton Dickinson).
