Angiogenin Expression during Early Human Placental Development; Association with Blood Vessel Formation

Figure 6

Angiogenin expression in decidua: maternal artery and macrophages. (A) Angiogenin immunoreactivity on frozen sections at week 7.5: (a) angiogenin specific polyclonal antibody (in red) highlighted vimentin-positive cells (in green): endothelial cells of the spiral artery (in yellow (red + green)) and sparse cells in the decidua (arrowhead). (b) Maternal macrophages (CD14 positive, in green) in a section of 11-week placenta were immunolabelled for angiogenin (in red), compared with the isotypic negative control (c). (B) In situ hybridisation on a frozen section of a 10-week placenta with a digoxigenin-labelled angiogenin cDNA probe revealing (in purple) angiogenin transcripts in spiral artery, glandular epithelium, and unidentified decidual cells (a). No signal was observed in RNase pretreated frozen sections (b). Counterstaining with Mayer’s haematoxylin. Bar, 20 μm. GL: glandular lumen and SAL: spiral artery lumen.