|
| Antibody specificity | Species, isotype | Concentration used | Cell specificity in placenta | Source |
|
| Angiogenin | Rabbit IgG
Mouse IgM
Clone MANG-1 |
6 μg/mL
6 μg/mL | Aim of the study |
[10]
Bachem (San Carlos, CA) |
| Cytokeratin 7 | Mouse IgG1
Clone OV-TL 12/30 | 2 μg/mL | Trophoblastic cells | Dako (Glostrup, Denmark) |
| Pan cytokeratin (5, 6, 8, 17, and probably 19) | Mouse IgG1
Clone MNF116 | 0.9 μg/mL | Epithelial glandular cells Trophoblastic cells | Dako |
| Vimentin | Mouse IgG1
Clone V9 | 5 μg/mL | Endothelial cells, mesenchymal cells, and vascular smooth muscle cells
Monocytes
Macrophages | Immunotech (Marseille, France) |
| Alpha smooth muscle actin | Mouse IgG2a
Clone 1A4 | Ascites fluid
1/300 | Vascular smooth muscle cells | Sigma ImmunoChemicals (St. Louis, MO) |
| Ki-67 | Mouse IgG1
Clone MIB-1 | 4 μg/mL | Proliferating cells | Immunotech |
| CD31, PECAM-1 (Platelet/endothelial cell adhesion molecule-1 ) | Mouse IgG1
Clone JC/70A | 4 μg/mL | Endothelial cells | Dako |
| CD34 | Mouse IgG1
Clone Qbend 10 | 4 μg/mL | Endothelial cells
Hematopoietic precursors | Immunotech |
| Tie-2 (Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains-2) | Rabbit IgG | 6 μg/mL | Endothelial cells | Santa Cruz Biotechnology (Santa Cruz, CA) |
VE-cadherin
(Vascular endothelial cadherin),
CD144, cadherin 5 | Mouse IgG1
Clone TEA1/31 | 4 μg/mL | Endothelial cells | Immunotech |
| VEGF-R2 (Flk-1) (Vascular endothelial growth factor receptor-2) | Mouse IgG1
Clone A3 | 4 μg/mL | Endothelial cells | Santa Cruz Biotechnology |
| vWF (von Willebrand factor) | Mouse IgG1 | 4 μg/mL | Endothelial cells | Roche Diagnostics (Meylan, France) |
| Epo-R (Erythropoietin receptor) | Rabbit IgG | 6 μg/mL | Endothelial cells
Erythroid precursors | Santa Cruz Biotechnology |
| CD14 | Mouse IgG2b
Clone MY4 | 6 μg/mL | Mature monocytes
Macrophages | Immunotech |
| CD45 (Leukocyte common antigen) | Mouse IgG1
Clone HI30 | 6 μg/mL | Leukocytes | BD Pharmingen (Le Pont-de-Claix, France) |
| Isotype control | Mouse IgG1 | | Nonspecific | Coulter Immunology (Hialeah, FL) |
| | Mouse IgG2a | | | Immunotech |
| | Mouse IgG2b | | | Coulter Immunology |
| | Mouse IgM | | | Coulter Immunology |
| | Rabbit IgG | | | Jackson ImmunoResearch Laboratories, Inc (West Grove, PA) |
| FITC-conjugated donkey anti-mouse IgG | Affinity Pure donkey IgG | 3.5 μg/mL | | Jackson ImmunoResearch |
| Texas Red-conjugated goat anti-mouse IgG (subclasses 1 + 2a + 2b + 3) | Affinity Pure goat IgG, Fc fragment specific | 3.5 μg/mL | | Jackson ImmunoResearch |
| FITC-conjugated goat anti-mouse IgM | Affinity Pure goat IgG, μ chain specific | 3.75 μg/mL | | Jackson ImmunoResearch |
| FITC-conjugated goat anti-rabbit IgG | Affinity Pure goat IgG, Fc fragment specific | 3.75 μg/mL | | Jackson ImmunoResearch |
| Rhodamine (TRITC-) labelled goat anti-rabbit IgG | Affinity Pure goat IgG, absorbed against human IgG | 4.4 μg/mL | | Sigma BioSciences |
| Texas Red-conjugated donkey anti-rabbit IgG | Affinity Pure donkey IgG | 3.5 μg/mL | | Jackson ImmunoResearch |
|
