A Functional Interplay between 5-Lipoxygenase and μ-Calpain Affects Survival and Cytokine Profile of Human Jurkat T Lymphocyte Exposed to Simulated Microgravity
Figure 3
Cytokine effects on Jurkat cell apoptosis under simulated microgravity conditions. Jurkat T cells were exposed (sim-μg) or not exposed (1 g) to simulated microgravity in absence (−) or in presence of the indicated cytokines (10 ng/mL), and DNA fragmentation (a), cytochrome c release (b), and μ-calpain activity (c) were evaluated as reported in Section 2. Results are reported as percentage of 1 g cells set to 100. For DNA fragmentation, 100% = 0.300 ± 0.030 ; for cytochrome c release, 100% = 0.074 ± 0.005; for μ-calpain activity, 100% = 66.26 ± 3.65 pmol/min per mg protein. denotes versus1 g cells; denotes versus sim-μg cells; denotes versus sim-μg cells.
