High Glucose Induces Sumoylation of Smad4 via SUMO2/3 in Mesangial Cells

Figure 6

Reverse transcription-PCR analysis of FN mRNA expression in mesangial cells. (a) Mesangial cells were treated with 30 mmol/L high glucose for 6, 12, and 24 h, and FN mRNA expression levels were detected by RT-PCR. ^∗𝑃<0.05 compared to normal control (NC) group. (b) Cells were treated with different concentrations of glucose or mannitol for 24 h, with 5.6 mmol/L glucose as a normal control and 30 mmol/L mannitol as an osmotic control. ^∗𝑃<0.05 compared to normal control; ^#𝑃<0.05 compared to mannitol control.

(a)

(b)