Biological Characterization and Pluripotent Identification of Sheep Dermis-Derived Mesenchymal Stem/Progenitor Cells
Figure 9
Neurogenic differentiation of DMS/PCs. After 10 days of induction, neural-like cells with a multipolar spindle-like shape were observed. Neural-specific markers including β-III tubulin (red), NF (red), nestin (green), and MAP-2 (green) were detected through immunofluorescence. (a) DMS/PCs morphology and identification of endothelial cells by immunofluorescent labeling. ((a1), (a2), (a3), and (a4)) Phase contrast. ((b1), (b2), (b3), and (b4)) β-III tubulin+, NF+, nestin+, and MAP-2+. ((c1), (c2), (c3), and (c4)) Merge (bar = 25 μm). (b) The cells in the control group did not express the neurogenic-specific makers. ((d1), (d2), (d3), and (d4)) Phase contrast. ((e1), (e2), (e3), and (e4)) β-III tubulin-, NF−, nestin−, and MAP-2−. ((f1), (f2), (f3), and (f4)) Merge (bar = 25 μm).
