Biological Characterization and Pluripotent Identification of Sheep Dermis-Derived Mesenchymal Stem/Progenitor Cells

Table 1

Primer information for DMS/PCs identification.

Gene	Primer sequence	Tm (°C)	Cycle	Fragment size (bp)
GAPDH	F: 5^′-GAAGGTCGGAGTGAACGGATT-3^′	60.0	30	517
	R: 5^′-GGTCATAAGTCCCTCCACGAT-3^′
𝛽-Integrin	F: 5^′-GTTTCACTTTGCTGGAGATGG-3′	58.0	30	243
	R: 5^′-GCAGATAATGTTCCTACCGCT-3^′
CD71	F: 5^′-GAGACTTCTTCCGTGCTACAT-3^′	62.0	30	723
	R: 5^′-GCTACCTTCCTCCTCCAACTC-3^′
CD44	F: 5^′-GTGTCGTGTGCCCAGTTATGA-3^′	60.0	30	511
	R: 5^′-CTCGTCAGAGGTCCCATTTTC-3^′
CD73	F: 5^′-GAGCAAGTGTGTCAACGCCAG-3^′	61.9	30	569
	R: 5^′-CATCCACGCCCTTCACTTTCT-3^′
PPAR-𝛾	F: 5^′-ATCTTCCAGGGGTGTCAGTTT-3^′	58.0	30	101
	R: 5^′-TGGTCATTCAAGTCAAGGTTC-3^′
LPL	F: 5^′-ACTATCCCCTGGGTAATGTGC-3^′	60.0	30	283
	R: 5^′-GGTTGGAAAGTGCCTCCGTTA-3^′
OPN	F: 5^′-TTTCACTCCACCTTTCCCTAC-3^′	58.5	30	489
	R: 5^′-CCACCCTGCTTTAACATATCC-3^′
COLI	F: 5^′-CCCAGTTGTCTTACGGCTATG-3^′	60.0	30	323
	R: 5^′-ACCTCTGTGTCCCTTCATTCC-3^′
ALP	F: 5^′-CGAAGCACAAGCACTCTCACT-3^′	60.0	30	419
	R: 5^′-GGGAGCCAGACCAAAGATAGA-3^′
COLII	F: 5^′-AAGTGGGGCGAGACTGTGATT-3^′	60.0	30	281
	R: 5^′-GGTTCTGGGTTCGGGTTTTTA-3^′