Homozygous Inactivating Mutation in NANOS3 in Two Sisters with Primary Ovarian Insufficiency
Figure 2
Detection of apoptosis using flow cytometry and Annexin-V staining in COS-1 cells transfected with wild type (WT) or p.Glu120Lys mutant (MUT) NANOS3-GFP, before and after induction of apoptosis with sodium butyrate (NaBu). Expression of mutant NANOS3 results in significantly higher apoptosis in comparison to wild type (). Error bars represent SEM for nine replicates; NT: nontransfected control.
