Rosiglitazone Regulates Anti-Inflammation and Growth Inhibition via PTEN

Figure 4

Effects of rosiglitazone on Akt phosphorylation in shLuc and shPTEN cells. Cells were treated with 25 𝜇M rosiglitazone prior to incubation with 5 𝜇g/mL LPS for 15 min. Cell lysates were harvested for determining the levels of phospho-Akt, Akt, and 𝛽-actin by Western blotting. Data shown are representative of three individual experiments. Data are expressed as mean ± SEM obtained from three individual cultures. ^∗𝑃< 0.05 compared with the control, untreated group. ^#𝑃 < 0.05 compared with the LPS group.