Optimization of Milk-Based Medium for Efficient Cultivation of Bifidobacterium pseudocatenulatum G4 Using Face-Centered Central Composite-Response Surface Methodology
Table 5
Experimental design and results using face-centered full factorial design (FCCD).
Run
Skim milk,
Yeast extract,
Responsesa
Coded value
Actual value
Coded value
Actual value
Max. biomass, (log10 cfu/mL)
β-Galactosidase production (U/mL)
Lactose balance (g/L)
FAN balance (mg/L)
1
−1
4.0
−1
1.0
5.15
0.19
4.59
198.24
2
−1
4.0
0
1.9
5.18
3.03
4.51
209.58
3
−1
4.0
+1
2.8
5.22
3.13
4.40
213.24
4
0
6.0
−1
1.0
7.09
7.06
10.83
229.15
5
0
6.0
0
1.9
7.15
8.37
11.53
237.06
6
0
6.0
0
1.9
7.11
9.40
11.58
231.04
7
0
6.0
0
1.9
7.12
9.32
11.81
234.65
8
0
6.0
0
1.9
7.14
8.35
11.91
238.98
9
0
6.0
0
1.9
7.16
9.38
11.84
233.14
10
0
6.0
+1
2.8
7.13
8.41
12.90
359.76
11
+1
8.0
−1
1.0
7.26
7.02
22.41
287.53
12
+1
8.0
0
1.9
7.23
6.27
19.10
290.59
13
+1
8.0
+1
2.8
7.11
6.18
25.25
318.70
All factorial and axial points are means of duplicate.
