Optimization of Milk-Based Medium for Efficient Cultivation of Bifidobacterium pseudocatenulatum G4 Using Face-Centered Central Composite-Response Surface Methodology

Table 8

ANOVA analysis for responses 𝑦₂ (β-galactosidase: U/mL), 𝑦₃ (lactose: g/L), and 𝑦₄ (FAN: mg/L).

Source	Sum squares	DF	Mean square	𝐹 value	𝑃 value
𝑦2
Model	96.50	5	19.30	52.91	<0.0001
Residual	2.55	7	0.36
Lack of fit	1.33	3	0.44	1.46	0.3521
Pure error	1.22	4	0.30
𝑅^2=0.9742
𝑦3
Model	151.33	2	75.66	615.28	<0.0001
Residual	1.23	10	0.12
Lack of fit	1.05	6	0.17	3.83	0.1075
Pure error	0.18	4	0.046
𝑅^2=0.9919
𝑦4
Model	14609.65	5	2921.93	123.58	0.0001
Residual	165.51	7	23.64
Lack of fit	126.17	3	42.06	4.28	0.0971
Pure error	39.34	4	9.84
𝑅^2=0.9888