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BioMed Research International
Volume 2014 (2014), Article ID 789591, 9 pages
http://dx.doi.org/10.1155/2014/789591
Research Article

A Proteomic View to Characterize the Effect of Chitosan Nanoparticle to Hepatic Cells: Is Chitosan Nanoparticle an Enhancer of PI3K/AKT1/mTOR Pathway?

1Instrument Technology Research Center, National Applied Research Laboratories, Hsinchu 300, Taiwan
2Translational Research Center, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan
3Department of Medical Research, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan
4Department of Obstetrics and Gynecology, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan
5School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
6Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan
7Department of Nuclear Medicine, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan
8Department of Chemistry, National Sun Yat-sen University, Kaohsiung 804, Taiwan
9National Sun Yat-sen University-Kaohsiung Medical University Joint Research Center, Kaohsiung 807, Taiwan
10Department of Biochemistry, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
11Department of Biomedical Engineering, National Yang-Ming University, Taipei 112, Taiwan
12Center of Biomedical Engineering and System Biology, Kaohsiung Medical University, Kaohsiung 807, Taiwan

Received 27 November 2013; Accepted 10 February 2014; Published 16 March 2014

Academic Editor: Yoshihiko Hayashi

Copyright © 2014 Ming-Hui Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Chitosan nanoparticle, a biocompatible material, was used as a potential drug delivery system widely. Our current investigation studies were the bioeffects of the chitosan nanoparticle uptake by liver cells. In this experiment, the characterizations of chitosan nanoparticles were measured by transmission electron microscopy and particle size analyzer. The average size of the chitosan nanoparticle was  nm, and the average zeta potential was  mV. Moreover, using proteomic approaches to analyze the differential protein expression patterns resulted from the chitosan nanoparticle uptaken by HepG2 and CCL-13 cells identified several proteins involved in the PI3K/AKT1/mTOR pathway. Our experimental results have demonstrated that the chitosan nanoparticle may involve in the liver cancer cell metastasis and proliferation.