Rapid Degradation of Hfq-Free RyhB in Yersinia pestis by PNPase Independent of Putative Ribonucleolytic Complexes
Figure 2
Effects of various ribonucleases on the transcriptional level of RyhB1 and RyhB2 upon inactivation of Hfq and RNase III. RyhB1 and RyhB2 were detected by Northern blotting using 5 μg of total RNA extracted from Y. pestis grown to exponential phase in BHI medium upon treatment with 100 μM DIP treatment for 20 min. Lanes 1–7 represent WT (lane 1), hfq mutant (lane 2), hfq-rnc double mutants (lane 3) and triple mutants lacking hfq, rnc, and another gene encoding RNase E910 (lane 4), RNase G (lane 5), PNPase (lane 6), or RNase II (lane 7).
