Correlation between Urothelial Differentiation and Sensory Proteins P2X3, P2X5, TRPV1, and TRPV4 in Normal Urothelium and Papillary Carcinoma of Human Bladder
Figure 2
Normal human urothelium (U). (a) Strong uroplakin (UP) immunolabeling (red) is restricted to umbrella cells. (b) Scanning electron microscopy (SEM) shows large polygonal umbrella cells with microridges on the urothelial apical surface. (c) Antibodies against P2X3 and (d) P2X5 label (red) all layers of the normal urothelium. The reaction is stronger in umbrella cells than in basal cells. (e) Anti-TRPV1 antibody labels (green) all urothelial cell layers. (f) Anti-TRPV4 labelling (green) is weak in basal and intermediate cell layers and moderate in umbrella cells. In (a), and (c)–(f) nuclei are labelled blue with DAPI. L = lumen, LP = lamina propria. Scale bars = 50 μm.
