Correlation between Urothelial Differentiation and Sensory Proteins P2X3, P2X5, TRPV1, and TRPV4 in Normal Urothelium and Papillary Carcinoma of Human Bladder
Figure 4
High grade papillary urothelial carcinomas (pT1 or pT2). (a) Uroplakin (UP) labelling (red) is negative. (b) Scanning electron microscopy (SEM) shows that superficial urothelial cells are small and polymorphic. They have microvilli on their apical surface. (c) Antibody against P2X3 weakly labels (green) urothelial cells. (d) P2X5 labelling (red) is present in all urothelial cells. Nuclei of some cells are also labelled. (e) TRPV1 labelling is negative. (f) Weak labelling of TRPV4 in urothelial cells (U) is seen, but strong TRPV4 labelling (arrows) is seen in the compartments of the lamina propria (LP). In images (a) and (c)–(f), nuclei are labelled blue with DAPI. L = lumen. Scale bars = 50 μm.
