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BioMed Research International
Volume 2014 (2014), Article ID 807196, 6 pages
First Trimester Biomarkers in the Prediction of Later Pregnancy Complications
1Department of Perinatal Medicine, The Royal Women’s Hospital, Cnr Grattan Street and Flemington Road, Parkville, VIC 3052, Australia
2Department of Obstetrics and Gynaecology, The University of Melbourne, Parkville, VIC 3010, Australia
3Monash Ultrasound for Women, 15 Murray Street, Clayton, VIC 3170, Australia
Received 14 December 2013; Accepted 27 February 2014; Published 30 March 2014
Academic Editor: Peter A. Fasching
Copyright © 2014 Stefan C. Kane et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Adverse obstetric outcomes, such as preeclampsia, preterm birth, gestational diabetes, and fetal growth restriction, are poorly predicted by maternal history and risk factors alone, especially in nulliparae. The ability to predict these outcomes from the first trimester would allow for the early initiation of prophylactic therapies, institution of an appropriate model and location of care, and recruitment of a truly “high risk” population to clinical trials of interventions to prevent or ameliorate these conditions. To this end, development of adequately sensitive and specific predictive tests for these outcomes has become a significant focus of perinatal research. This paper reviews the biomarkers involved in these multiparametric tests and also outlines the performance of these tests and issues regarding their introduction into clinical practice.
It is common practice for pregnancies to be predictively categorised as “low” or “high” risk, based on the perceived likelihood of an adverse neonatal or maternal outcome. The dichotomous simplicity of such a categorisation is immediately appealing but fails to reflect adequately the spectrum of risk that exists for all pregnant patients and does not acknowledge the limited clinical utility of current strategies for the prediction of obstetric risk, especially among nulliparae. Routine antenatal investigations generally aim to identify concurrent conditions of obstetric relevance, such as thalassaemia, anaemia, and vertically transmissible maternal infections, such as syphilis . First trimester screening for fetal aneuploidy represents the most commonly employed test in early gestation for the prediction of a later pregnancy complication, namely, the delivery of an infant with a chromosomal anomaly. The principles that underpin these multiparametric tests for aneuploidy have informed the recent development of screening strategies using multiple biomarkers in early gestation for the prediction of other later pregnancy complications , such as preeclampsia, spontaneous preterm birth, gestational diabetes, and fetal growth restriction. In addition to outlining the rationale for predictive testing in the first trimester, this paper aims to review the composition and performance of testing strategies for these four entities, which are cumulatively responsible for a significant proportion of adverse perinatal and maternal outcomes. For the purpose of this review, a biomarker is considered to be any objectively measured and evaluated characteristic that reflects normal or pathogenic biological processes .
2. The Rationale for Screening
The capacity in early gestation to predict later pregnancy complications allows for(i)the early commencement of proven prophylactic therapies (pharmacological and otherwise) to reduce the risk of the adverse outcome in question;(ii)institution of an appropriate model of antenatal care and level of clinical surveillance;(iii)recruitment of a truly “high risk” population to trials of interventions intended to prevent or mitigate specific adverse outcomes.
Pregnancy is a domain in which past outcomes do, to a large extent, predict future risk. Among the strongest risk factors for preeclampsia, preterm birth, gestational diabetes, and fetal growth restriction is a history of these conditions in a prior gestation, as outlined in Table 1.
However, such information is of little benefit to a first-time mother’s pregnancy complicated by these adverse outcomes, which may have resulted in significant maternal and perinatal morbidity, if not mortality. Trying to predict such adverse events on the basis of maternal factors alone is of limited utility in primigravidae. For example, for the prediction of preeclampsia in nulliparae, an algorithm incorporating maternal factors such as body mass index, mean arterial pressure, and family history yields only a 37% detection rate for a 10% false positive rate . These limitations have prompted research into more sophisticated predictive tests for adverse pregnancy outcome.
Abnormal levels of maternal serum analytes assessed in conventional aneuploidy screening have long been acknowledged to have a statistically significant association with adverse obstetric outcome in euploid pregnancies. For instance, a pregnancy-associated plasma protein A (PAPP-A) of less than 0.42 multiples of the median (i.e., less than the 5th centile) in the first trimester has an adjusted odds ratio of 2.81 (95% CI 2.35–3.35) for low birth weight (less than the 5th centile) . However, its sensitivity is only 12.23%, with a positive predictive value of 9.5%. Indeed, no later pregnancy complication can be predicted with sufficient specificity and sensitivity by any single biomarker. Multiparametric testing—the assessment of multiple parameters in combination (including baseline maternal characteristics)—is required to achieve adequate predictive performance, as is the case with first trimester combined screening for aneuploidy . Table 2 summarises the best-performing multiparametric tests for the common pregnancy complications reviewed in this paper.
Preeclampsia is the commonest serious medical disorder of pregnancy, with a worldwide incidence of 2–8% . The utility of a wide range of biomarkers in predicting this outcome has been investigated, including(i)maternal mean arterial pressure [20, 21];(ii)uterine artery Doppler indices [22, 23];(iii)markers of placental function, such as PAPP-A and plasma protein 13 (PP13) ;(iv)other proteins of placental origin, including inhibin A  and activin A ;(v)angiogenic agents, such as placental growth factor (PlGF) and vascular endothelial growth factor (VEGF) , and their inhibitors soluble fms-like tyrosine kinase-1 (sFlt-1)  and soluble endoglin (sEng) .
Only in combination do any of these biomarkers offer sufficient sensitivity and specificity to be of clinical utility in the prediction of preeclampsia. In 2009, researchers from the Fetal Medicine Foundation (UK) evaluated a multiparametric test incorporating maternal factors, mean arterial pressure, uterine artery Doppler pulsatility index, placental growth factor (PlGF), and PAPP-A in 7797 singleton pregnancies. It detected 93% of early-onset preeclampsia for a false positive rate (FPR) of 5% . This approach has recently been validated in an Australian population (), in which 91.7% of early-onset preeclampsia was detected for a FPR of 10% , and in Spain (), with an 80.8% detection rate for early preeclampsia at a 10% FPR . Some institutions have now introduced this testing strategy into clinical practice and are conducting it at the same time as conventional first trimester aneuploidy screening. Interestingly, just as cell-free fetal DNA in maternal serum is becoming the new “gold standard” screening test for aneuploidy, so too has it been found to have value in predicting preeclampsia, with a recent small retrospective case-control study () demonstrating a sensitivity and specificity of 100% for the development of this condition .
The clinical utility of these predictive tests for preeclampsia is enhanced by the potential availability of prophylactic therapies to ameliorate the condition. Low-dose aspirin (75–100 mg daily) has been shown to reduce the risk of preeclampsia, especially if started in early pregnancy (16 weeks) . Its capacity to do so among those predicted to be at high risk of this condition on the basis of predictive tests is the subject of ongoing randomised trials . The only other therapy proven to reduce the risk of preeclampsia is calcium , although many other agents are under investigation, including low-molecular-weight heparin , high-dose folate , vitamin D , and statins .
4. Preterm Birth
Spontaneous preterm labour accounts for around 60–70% of all preterm deliveries and thus makes a significant contribution to perinatal morbidity and mortality . The disparate pathogenic processes that result in preterm labour are incompletely understood , and as such, its prediction remains challenging. Meta-analyses of 116 biomarkers studied over the last four decades , and 30 novel biomarkers investigated over the last ten years  have concluded that none perform sufficiently in predicting preterm birth to be clinically useful. Similarly, attempts to devise multiparametric models, incorporating biomarkers such as PAPP-A, PP13, and uterine artery Doppler indices in the first trimester, perform as well as or only marginally better than risk prediction based on maternal characteristics alone [42, 43]. Incorporating measurement of cervical length at 11–13 weeks may improve predictive performance, with one such model predicting 54.8% of all spontaneous preterm births earlier than 34 weeks (at a FPR of 10%) when evaluated in 9974 pregnancies , although others have not replicated these findings [44, 45]. This is in contrast to cervical assessment in the second trimester, which has consistently been shown to aid in the prediction of preterm birth [46, 47].
Similarly, current strategies to prevent early spontaneous delivery are limited in scope and effect . Apart from modification of lifestyle factors, therapies with the most evidence include vaginal progesterone supplementation  and cervical cerclage . These interventions have mostly been studied in women with a history of preterm birth and/or with a short cervix in the midtrimester. Until the pathogenic pathways for preterm labour are better understood, improved prediction and thus prevention of this outcome will remain an enigma , especially for nulliparae with no a priori risk factors, and may prove to be optimally instituted in the second rather than first trimester.
5. Gestational Diabetes
The worldwide incidence of gestational diabetes mellitus (GDM) is rising , and although controversy continues regarding optimal diagnostic thresholds , there is clear evidence that its identification and treatment optimise perinatal outcomes . Numerous risk factors for gestational diabetes are well established, including maternal BMI, advancing age, cultural background, history of polycystic ovarian syndrome, and family history of diabetes , although some develop impaired glucose tolerance in the absence of any identifiable risk factor. Accurate early prediction of those destined to develop gestational diabetes would allow for the early initiation of measures that may prevent or ameliorate the effects of this condition, such as exercise programs  and dietary modifications , although further research is required to confirm the specific benefits of these early interventions.
Elevated fasting blood sugar levels (within the range of normoglycaemia) in the first trimester are independently associated with the later development of gestational diabetes, with fasting glucose cut-off levels of 80–85 mg/dL yielding sensitivities of 75–55% and specificities of 52–75% for GDM prediction in one study () . Other biomarkers shown to be predictive for GDM include sex hormone binding globulin (SHBG), highly sensitive C-reactive protein (CRP), and adiponectin. A model incorporating SHBG and CRP assessed prior to 15 weeks () predicted GDM with sensitivity and specificity of 74.07% and 75.62%, respectively, with an overall accuracy of 75.46% . An alternative model, using adiponectin and SHBG in addition to maternal characteristics, demonstrated similar predictive performance in a case-control study of 380 women: 74.1% detection for a 20% false positive rate, compared with 61.6% detection using maternal characteristics alone .
6. Fetal Growth Restriction
A growth-restricted fetus is one that has failed to reach its growth potential. Many, but not all, of such fetuses will be small for gestational age: less than the 10th centile on population or customised growth charts, depending on local policy. Growth-restricted infants are overrepresented in perinatal morbidity and mortality statistics  and have increased lifetime risks of cardiovascular and metabolic disease . Fetal growth restriction (FGR) can arise on account of maternal, placental, or fetal factors, many of which are unmodifiable (e.g., maternal age), or cannot be modified once pregnancy is established. As such, apart from lifestyle modifications such as smoking cessation, few interventions have been identified that can prevent or mitigate the effects of FGR in gravida predicted to be at high risk of the same. Low-dose aspirin (75–150 mg daily) has demonstrated benefit in preventing growth restriction in the absence of preeclampsia when commenced in early gestation: a meta-analysis comparing aspirin commenced at <16 weeks and >16 weeks found a significant difference in relative risk of FGR between the two groups ( (95% CI 0.33–0.64) versus 0.98 (95% CI 0.88–1.08), ) . At present, the primary benefit arising from the early prediction of growth restriction is likely to be the adoption of a model of obstetric care that facilitates appropriate clinical and sonographic surveillance.
Given the putative similarities in the placental origins of some aspects of FGR and preeclampsia, it is not surprising that many biomarkers are common to the prediction of both. Doppler analysis of the uterine artery in the first trimester is significantly different in pregnancies destined to develop FGR, with a recent prospective study identifying a correlation between the lowest uterine artery pulsatility index value and subsequent birthweight in an unselected population . Ultrasound can also be used to estimate placental volume in the first trimester, which is significantly smaller in FGR pregnancies .
As always, multiparametric testing strategies achieve the best predictive performance. Almost three-quarters (73%) of fetal growth restriction requiring delivery prior to 37/40 was identified in a study () using a first trimester screening algorithm incorporating maternal factors and numerous biomarkers, including fetal nuchal translucency (NT) thickness, serum pregnancy-associated plasma protein-A (PAPP-A), free beta-human chorionic gonadotrophin (beta-hCG), mean arterial pressure (MAP), uterine artery pulsatility index (PI), placental growth factor (PlGF), placental protein 13 (PP13), and A disintegrin and metalloprotease (ADAM12) . A subsequently proposed model using a more pragmatic number of biomarkers (maternal characteristics, uterine artery pulsatility index, MAP, PAPP-A, and PlGF) achieved a detection rate for FGR requiring delivery 37 weeks of 55.5% for a 10.9% FPR in a study group of 62052 pregnant women .
7. Future Directions
The application of these predictive tests to the general population of pregnant women is an example of screening. It is crucial that the principles of screening , established in the 1960s and unchanged since, be applied to this domain. In particular, there must be interventions proven to improve outcomes for women and their babies who are screened to be at “high risk” of specific pregnancy complications. Patient and clinician acceptability would be enhanced through the delivery of these tests in an integrated model with well documented performance characteristics and would ideally involve screening for aneuploidy as well. The cost- effectiveness of these tests must be clearly established, and robust economic modelling will be required to justify the allocation of limited public healthcare resources to them. If the costs of these tests are to be borne privately, they run the risk of promoting further health inequity, particularly among those who live some distance from tertiary-level obstetric care, for whom these tests may be of significant potential benefit. Furthermore, the potential impact of commercial imperatives on the timing and clinical context of the introduction of these tests should be acknowledged, anticipated, and managed appropriately.
As with any screening program, participation should be voluntary, and clinicians must remain comfortable caring for patients who exercise their right “not to know” and decline any or all of these screening tests. Finally, the emotional and psychological impact of a “high risk” result can be substantial , whether the predicted outcome eventuates or not, and it is important that appropriate resources are in place to support those screened to be high risk.
Once these concerns are addressed, predictive testing strategies in early pregnancy could finally offer a clinically meaningful and accurate distinction between “low” and “high” risk pregnancies, with improved outcomes for both as a result.
Conflict of Interests
The authors declare that there is no conflict of interests regarding the publication of this paper.
- NHS National Institute for Health and Clinical Excellence, “Antenatal care,” NICE Clinical Guideline 62, London, UK, 2010.
- H. S. Cuckle, “Screening for pre-eclampsia—lessons from aneuploidy screening,” Placenta, vol. 32, pp. S42–S48, 2011.
- J. Atkinson A.J., W. A. Colburn, V. G. DeGruttola et al., “Biomarkers and surrogate endpoints: preferred definitions and conceptual framework,” Clinical Pharmacology and Therapeutics, vol. 69, no. 3, pp. 89–95, 2001.
- K. Duckitt and D. Harrington, “Risk factors for pre-eclampsia at antenatal booking: systematic review of controlled studies,” British Medical Journal, vol. 330, no. 7491, pp. 565–567, 2005.
- M. S. Esplin, E. O'Brien, A. Fraser et al., “Estimating recurrence of spontaneous preterm delivery,” Obstetrics and Gynecology, vol. 112, no. 3, pp. 516–523, 2008.
- A. Z. Khambalia, J. B. Ford, N. Nassar, et al., “Occurrence and recurrence of diabetes in pregnancy,” Diabetic Medicine, vol. 30, no. 4, pp. 452–456, 2013.
- B. J. Voskamp, B. M. Kazemier, A. C. Ravelli, et al., “Recurrence of small-for-gestational-age pregnancy: analysis of first and subsequent singleton pregnancies in The Netherlands,” American Journal of Obstetrics & Gynecology, vol. 208, no. 5, Article ID 374.e1, 2013.
- L. C. Y. Poon, N. A. Kametas, N. Maiz, R. Akolekar, and K. H. Nicolaides, “First-trimester prediction of hypertensive disorders in pregnancy,” Hypertension, vol. 53, no. 5, pp. 812–818, 2009.
- F. J. Park, C. H. Leung, L. C. Poon, et al., “Clinical evaluation of a first trimester algorithm predicting the risk of hypertensive disease of pregnancy,” Australian and New Zealand Journal of Obstetrics and Gynaecology, vol. 53, no. 6, pp. 532–539, 2013.
- E. Scazzocchio, F. Figueras, F. Crispi, et al., “Performance of a first-trimester screening of preeclampsia in a routine care low-risk setting,” American Journal of Obstetrics & Gynecology, vol. 208, pp. 203.e1–203.e10, 2013.
- E. Greco, R. Gupta, A. Syngelaki, L. C. Y. Poon, and K. H. Nicolaides, “First-trimester screening for spontaneous preterm delivery with maternal characteristics and cervical length,” Fetal Diagnosis and Therapy, vol. 31, no. 3, pp. 154–161, 2012.
- A. M. Maged, G. A. Moety, W. A. Mostafa, and D. A. Hamed, “Comparative study between different biomarkers for early prediction of gestational diabetes mellitus,” Journal of Maternal-Fetal and Neonatal Medicine, 2013.
- S. Nanda, M. Savvidou, A. Syngelaki, R. Akolekar, and K. H. Nicolaides, “Prediction of gestational diabetes mellitus by maternal factors and biomarkers at 11 to 13 weeks,” Prenatal Diagnosis, vol. 31, no. 2, pp. 135–141, 2011.
- G. Karagiannis, R. Akolekar, R. Sarquis, D. Wright, and K. H. Nicolaides, “Prediction of small-for-gestation neonates from biophysical and biochemical markers at 11–13 weeks,” Fetal Diagnosis and Therapy, vol. 29, no. 2, pp. 148–154, 2011.
- L. C. Poon, A. Syngelaki, R. Akolekar, et al., “Combined screening for preeclampsia and small for gestational age at 11–13 weeks,” Fetal Diagnosis and Therapy, vol. 33, no. 1, pp. 16–27, 2013.
- R. A. North, L. M. E. McCowan, G. A. Dekker et al., “Clinical risk prediction for pre-eclampsia in nulliparous women: development of model in international prospective cohort,” British Medical Journal, vol. 342, no. 7803, Article ID d1875, 2011.
- L. Dugoff, J. C. Hobbins, F. D. Malone et al., “First-trimester maternal serum PAPP-A and free-beta subunit human chorionic gonadotropin concentrations and nuchal translucency are associated with obstetric complications: A population-based screening study (The FASTER Trial),” American Journal of Obstetrics and Gynecology, vol. 191, no. 4, pp. 1446–1451, 2004.
- K. H. Nicolaides, “Turning the pyramid of prenatal care,” Fetal Diagnosis and Therapy, vol. 29, no. 3, pp. 183–196, 2011.
- L. Duley, “The global impact of pre-eclampsia and eclampsia,” Seminars in Perinatology, vol. 33, no. 3, pp. 130–137, 2009.
- J. S. Cnossen, K. C. Vollebregt, N. De Vrieze et al., “Accuracy of mean arterial pressure and blood pressure measurements in predicting pre-eclampsia: systematic review and meta-analysis,” BMJ, vol. 336, no. 7653, pp. 1117–1120, 2008.
- L. C. Y. Poon, N. A. Kametas, I. Pandeva, C. Valencia, and K. H. Nicolaides, “Mean arterial pressure at 11 + 0 to 13 + 6 weeks in the prediction of preeclampsia,” Hypertension, vol. 51, no. 4, pp. 1027–1033, 2008.
- J. S. Cnossen, R. K. Morris, G. Ter Riet et al., “Use of uterine artery Doppler ultrasonography to predict pre-eclampsia and intrauterine growth restriction: a systematic review and bivariable meta-analysis,” Canadian Medical Association Journal, vol. 178, no. 6, pp. 701–711, 2008.
- L. Velauthar, J. Zamora, J. Aquilina, et al., “Prediction of pre-eclampsia using first trimester uterine artery Doppler: a meta-analysis of 43 122 pregnancies,” Ultrasound in Obstetrics & Gynecology, vol. 40, supplement 1, p. 49, 2012.
- K. Spencer, N. J. Cowans, I. Chefetz, J. Tal, and H. Meiri, “First-trimester maternal serum PP-13, PAPP-A and second-trimester uterine artery Doppler pulsatility index as markers of pre-eclampsia,” Ultrasound in Obstetrics and Gynecology, vol. 29, no. 2, pp. 128–134, 2007.
- L. J. Salomon, C. Benattar, F. Audibert et al., “Severe preeclampsia is associated with high inhibin A levels and normal leptin levels at 7 to 13 weeks into pregnancy,” American Journal of Obstetrics and Gynecology, vol. 189, no. 6, pp. 1517–1522, 2003.
- C. Y. T. Ong, A. W. Liao, S. Munim, K. Spencer, and K. H. Nicolaides, “First-trimester maternal serum activin a in pre-eclampsia and fetal growth restriction,” Journal of Maternal-Fetal and Neonatal Medicine, vol. 15, no. 3, pp. 176–180, 2004.
- A.-K. Wikström, A. Larsson, U. J. Eriksson, P. Nash, S. Nordén-Lindeberg, and M. Olovsson, “Placental growth factor and soluble FMS-like tyrosine kinase-1 in early-onset and late-onset preeclampsia,” Obstetrics and Gynecology, vol. 109, no. 6, pp. 1368–1374, 2007.
- R. Akolekar, J. De Cruz, J.-M. Foidart, C. Munaut, and K. H. Nicolaides, “Maternal plasma soluble fms-like tyrosine kinase-1 and free vascular endothelial growth factor at 11 to 13 weeks of gestation in preeclampsia,” Prenatal Diagnosis, vol. 30, no. 3, pp. 191–197, 2010.
- J.-M. Foidart, C. Munaut, F. Chantraine, R. Akolekar, and K. H. Nicolaides, “Maternal plasma soluble endoglin at 11-13 weeks' gestation in preeclampsia,” Ultrasound in Obstetrics and Gynecology, vol. 35, no. 6, pp. 680–687, 2010.
- N. Papantoniou, V. Bagiokos, K. Agiannitopoulos, et al., “RASSF1A in maternal plasma as a molecular marker of preeclampsia,” Prenatal Diagnosis, vol. 33, pp. 682–687, 2013.
- S. Roberge, K. H. Nicolaides, S. Demers, et al., “Prevention of perinatal death and adverse perinatal outcome using low-dose aspirin: a meta-analysis,” Ultrasound in Obstetrics & Gynecology, vol. 41, no. 5, pp. 491–499, 2013.
- “The ASPRE trial: combined multi-marker screening and randomised patient treatment with Aspirin for evidence-based PRE-eclampsia prevention,” International Standard Randomised Controlled Trials Number 10.1186/ISRCTN13633058.
- G. J. Hofmeyr, T. A. Lawrie, A. N. Atallah, and L. Duley, “Calcium supplementation during pregnancy for preventing hypertensive disorders and related problems,” Cochrane Database of Systematic Reviews, no. 8, Article ID CD001059, 2010.
- “The EPPI Trial—Enoxaparin for the Prevention of Preeclampsia and Intrauterine growth restriction—a pilot randomised open-label trial,” ACTRN12609000699268.
- “Effect of Folic Acid Supplementation in Pregnancy on Preeclampsia—Folic Acid Clinical Trial (FACT),” NCT01355159.
- “Prevention of Adverse Pregnancy Outcome with Vitamin D Supplementation During Pregnancy,” NCT01418664.
- “Pravastatin for the Prevention of Preeclampsia in High-Risk Women: A Phase I Pilot Study,” NCT01717586.
- R. Rattihalli, L. Smith, and D. Field, “Prevention of preterm births: are we looking in the wrong place? The case for primary prevention,” Archives of Disease in Childhood: Fetal and Neonatal Edition, vol. 97, pp. F160–F161, 2012.
- C. Voltolini, M. Torricelli, N. Conti, et al., “Understanding spontaneous preterm birth: from underlying mechanisms to predictive and preventive interventions,” Reproductive Sciences, vol. 20, no. 11, pp. 1274–1292, 2013.
- R. Menon, M. R. Torloni, C. Voltolini et al., “Biomarkers of spontaneous preterm birth: an overview of the literature in the last four decades,” Reproductive Sciences, vol. 18, no. 11, pp. 1046–1070, 2011.
- A. Conde-Agudelo, A. T. Papageorghiou, S. H. Kennedy, and J. Villar, “Novel biomarkers for the prediction of the spontaneous preterm birth phenotype: a systematic review and meta-analysis,” British Journal of Obstetrics and Gynaecology, vol. 118, no. 9, pp. 1042–1054, 2011.
- M. J. Stout, K. R. Goetzinger, M. G. Tuuli, et al., “First trimester serum analytes, maternal characteristics and ultrasound markers to predict pregnancies at risk for preterm birth,” Placenta, vol. 34, no. 1, pp. 14–19, 2013.
- J. Beta, R. Akolekar, W. Ventura, A. Syngelaki, and K. H. Nicolaides, “Prediction of spontaneous preterm delivery from maternal factors, obstetric history and placental perfusion and function at 11–13 weeks,” Prenatal Diagnosis, vol. 31, no. 1, pp. 75–83, 2011.
- M. Parra-Cordero, A. Sepúlveda-Martínez, G. Rencoret, et al., “Is there a role for cervical assessment and uterine artery Doppler performed during the first trimester of pregnancy as a screening test for spontaneous preterm delivery?” Ultrasound in Obstetrics & Gynecology, 2013.
- N. Sananes, N. Meyer, A. Gaudineau, et al., “Prediction of spontaneous preterm delivery in the first trimester of pregnancy,” European Journal of Obstetrics & Gynecology and Reproductive, vol. 171, no. 1, pp. 18–22, 2013.
- E. Celik, M. To, K. Gajewska, G. C. S. Smith, and K. H. Nicolaides, “Cervical length and obstetric history predict spontaneous preterm birth: development and validation of a model to provide individualized risk assessment,” Ultrasound in Obstetrics and Gynecology, vol. 31, no. 5, pp. 549–554, 2008.
- J. D. Iams, “Clinical practice. Prevention of preterm parturition,” The New England Journal of Medicine, vol. 370, no. 3, pp. 254–261, 2014.
- S. S. Hassan, R. Romero, D. Vidyadhari et al., “Vaginal progesterone reduces the rate of preterm birth in women with a sonographic short cervix: a multicenter, randomized, double-blind, placebo-controlled trial,” Ultrasound in Obstetrics and Gynecology, vol. 38, no. 1, pp. 18–31, 2011.
- V. Berghella, “Progesterone and preterm birth prevention: translating clinical trials data into clinical practice,” American Journal of Obstetrics and Gynecology, vol. 206, no. 5, pp. 376–386, 2012.
- L. J. Muglia and M. Katz, “The enigma of spontaneous preterm birth,” The New England Journal of Medicine, vol. 362, no. 6, pp. 484–535, 2010.
- D. Dabelea, J. K. Snell-Bergeon, C. L. Hartsfield, K. J. Bischoff, R. F. Hamman, and R. S. McDuffie, “Increasing prevalence of gestational diabetes mellitus (GDM) over time and by birth cohort: kaiser Permanente of Colorado GDM screening program,” Diabetes Care, vol. 28, no. 3, pp. 579–584, 2005.
- R. G. Moses, G. J. Morris, P. Petocz, F. Sangil, and D. Garg, “The impact of potential new diagnostic criteria on the prevalence of gestational diabetes mellitus in Australia,” Medical Journal of Australia, vol. 194, no. 7, pp. 338–340, 2011.
- HAPO Study Cooperative Research Group, “Hyperglycemia and adverse pregnancy outcomes,” The New England Journal of Medicine, vol. 358, no. 19, pp. 1991–2002, 2008.
- C. G. Solomon, W. C. Willett, V. J. Carey et al., “A prospective study of pregravid determinants of gestational diabetes mellitus,” Journal of the American Medical Association, vol. 278, no. 13, pp. 1078–1083, 1997.
- D. K. Tobias, C. Zhang, R. M. Van Dam, K. Bowers, and F. B. Hu, “Physical activity before and during pregnancy and risk of gestational diabetes mellitus: a meta-analysis,” Diabetes Care, vol. 34, no. 1, pp. 223–229, 2011.
- B. Karamanos, A. Thanopoulou, E. Anastasiou, et al., “Relation of the Mediterranean diet with the incidence of gestational diabetes,” European Journal of Clinical Nutrition, vol. 68, no. 1, pp. 8–13.
- S. Riskin-Mashiah, A. Damti, G. Younes, and R. Auslender, “First trimester fasting hyperglycemia as a predictor for the development of gestational diabetes mellitus,” European Journal of Obstetrics Gynecology and Reproductive Biology, vol. 152, no. 2, pp. 163–167, 2010.
- D. D. Mcintire, S. L. Bloom, B. M. Casey, and K. J. Leveno, “Birth weight in relation to morbidity and mortality among newborn infants,” The New England Journal of Medicine, vol. 340, no. 16, pp. 1234–1238, 1999.
- D. J. P. Barker, J. G. Eriksson, T. Forsén, and C. Osmond, “Fetal origins of adult disease: Strength of effects and biological basis,” International Journal of Epidemiology, vol. 31, no. 6, pp. 1235–1239, 2002.
- S. Roberge, K. H. Nicolaides, S. Demers, et al., “Prevention of perinatal death and adverse perinatal outcome using low-dose aspirin: a meta-analysis,” Ultrasound in Obstetrics & Gynecology, vol. 41, no. 5, pp. 491–499, 2013.
- A. Sarmiento, A. Casasbuenas, N. Rodriguez, et al., “First-trimester uterine artery Doppler velocimetry in the prediction of birth weight in a low-risk population,” Prenat Diagn, vol. 33, no. 1, pp. 21–24, 2013.
- S. L. Collins, G. N. Stevenson, and J. A. Noble, “Impey L. Rapid calculation of standardized placental volume at 11 to 13 weeks and the prediction of small for gestational age babies,” Ultrasound in Medicine & Biology, vol. 39, no. 2, pp. 253–260, 2013.
- J. M. G. Wilson and G. Jungner, Principles and Practice of Screening for Disease, Public health papers no. 34, World Health Organization, Geneva, Switzerland, 1968.
- J. M. Harris, L. Franck, and S. Michie, “Assessing the psychological effects of prenatal screening tests for maternal and foetal conditions: a systematic review,” Journal of Reproductive and Infant Psychology, vol. 30, pp. 222–246, 2013.