ATF4- and CHOP-Dependent Induction of FGF21 through Endoplasmic Reticulum Stress

Figure 1

ER stress increases FGF21 mRNA levels. (a) 3T3-L1 adipocytes were treated with TG (100 nM) for 0, 2, 4, 8, 16, and 24 h; (b) 3T3-L1 adipocytes were treated with TG (25, 50, and 100 nM) for 16 h. Total cellular RNA was isolated. The mRNA levels of ATF4, XBP1-sp, CHOP, and FGF21 were measured by real-time RT-PCR. Values are mean ± S.E. of three independent experiments. Statistical significance relative to vehicle control: ^∗𝑃<0.05; ^∗∗𝑃<0.01; ^∗∗∗𝑃<0.001.

(a)

(b)