Uncoupling Protein 2 Regulates Palmitic Acid-Induced Hepatoma Cell Autophagy

Figure 2

The effects of PA on UCP2 expression. H4IIE cells were treated with PA (250 μM) conjugated to fatty acid-free BSA for 2, 4, 6, 8, 12, and 24 h as indicated. The mRNA level of UCP2 was normalized to 18 s. This ratio was set as 100% with respect to the BSA control. The protein level of UCP2 was normalized to 𝛽-actin, and this ratio was set as 100% with respect to the BSA control. Data are expressed as the mean ± SD of three independent experiments each performed in triplicate.