Uncoupling Protein 2 Regulates Palmitic Acid-Induced Hepatoma Cell Autophagy
Figure 6
UCP2- and PA-mediated ATP synthesis. (a) H4IIE cells were transfected with UCP2 plasmid (UCP2-Tr) or control vector (Vector-Tr). ATP induced by 250 μM PA for 6 h was assessed using CellTiter-Glo2.0 assay kit, and p-AMPK was normalized to -actin. This ratio was set to 100% in the control of BSA. (b) H4IIE cells were transfected with UCP2 siRNA (UCP2-siRNA) or scrambled siRNA (scramble) for 72 h to inhibit the expression of UCP2 and treated with PA. ATP induced by 250 μM PA for 6 h was assessed using CellTiter-Glo2.0 assay kit, and p-AMPK was normalized to -actin. This ratio was set to 100% in the control of BSA. Data are expressed as the mean ± SD for each experiment. All data presented are representative of three separate experiments with consistent results.
