Novel Study on N-Nitrosamines as Risk Factors of Cardiovascular Diseases

Table 2

Changes in the activities of superoxide dismutase, GSH-R, GST, paraoxonase, catalase, glutathione peroxidase and levels of both GSH and free radicals after treatment of male rats with a daily doses of dibuty-, ethylbutyl-, butylpropyl-, diethyl-, dimethyl- and diphenylnitrosamine for two weeks.

	Control	NDBA	NEBA	NBPA	NDEA	NDMA	NDPA
GSH content; mole/gm liver	1.8165 ± 0.39	2.268 ± 0.724; (+25%, )*	2.184 ± 0.11; (+20%, )*	2.227 ± 0.836; (+23%, )*	2.21 ± 0.10; (+22%, )*	2.516 ± 0.682; (+39%, )*	2.091 ± 0.49; (+15%, )*
Free Radicals (TBARS); mole/gm liver	5.481 ± 0.35	10.856 ± 1.24; (+98%, )*	8.35 ± 0.531; (+52%, )*	7.952 ± 0.421; (+45%, )*	7.552 ± 0.865; Ns	12.68 ± 1.427; (+131%, )*	7.618 ± 0.42; Ns
GSH-R activity; nmolNADPH/mgprotein/min	135 ± 5.12	269 ± 8.75; (+100%, )*	194 ± 8.4; (+44%, )*	181 ± 10.92; (+34%, )*	208 ± 7.22; (+55%, )*	208 ± 13.84; (+54%, )*	139.39 ± 7.22; Ns
GST activity; mmole/mgprotein/min	0.335 ± 0.008	0.515 ± 0.023; (+54%, )*	0.536 ± 0.016; (+60%, )*	0.364 ± 0.199; Ns	0.506 ± 0.027; (+51%, )*	0.361 ± 0.013; Ns	0.215 ± 0.006; (−36%, )*
Superoxide dismutase; Units/g hemoglobin	1.603 ± 0.231	0.837 ± 0.186; (−48%, )*	1.460 ± 0.209; Ns	0.769 ± 0.096; (−52%, )*	1.985 ± 0.065; Ns	1.20 ± 0.194; Ns	0.772 ± 0.161; (−52%, )*
Paraoxonase activity; mole Paraoxonhydrolyzed/mg protein/min	8.249 ± 0.600	9.527 ± 1.11; Ns	15.173 ± 1.469; (+84%, )*	11.577 ± 0.823; (+40%, )*	14.90 ± 0.773; (+81%, )*	18.234 ± 0.584; (+121%, )*	12.22 ± 0.797; (+48%, )*
Catalase (U/gm tissue)	3.05 ± 0.32	7.01 ± 0.56; (+131%, )	8.14 ± 0.43; (+169%, )	2.39 ± 0.18; NS	2.02 ± 0.22; NS	13.4 ± 0.61; (339%, )	2.86 ± 0.36; NS
Glutathione peroxidase; IU/gm Wet tissue	69.29 ± 1.94	21.7 ± 1.11; (−69%, )*	20.41 ± 0.99; (−71%, )*	21.04 ± 0.6; (−70%, )*	29.8 ± 0.75; (−57%, )*	39.83 ± 2.26; (−43%, )*	59 ± 2.93; (−15%, )*

Values are expressed as Mean ± SEM.
NS: values are not significant statistically compared to the control group where .
*Values are significantly differentcompared to control group where .