Accumulation of Extracellular Hyaluronan by Hyaluronan Synthase 3 Promotes Tumor Growth and Modulates the Pancreatic Cancer Microenvironment
Figure 3
Pancreatic cancer xenograft tumors overexpressing HAS3 grow faster and respond better to hyaluronan removal than HAS2 or parental tumors. To compare in vivo growth, BxPC-3, BxPC-3 HAS2, and BxPC-3 HAS3 tumor cells were inoculated adjacent to the tibial periosteum in the hind limb of nu/nu mice (/group), and tumor growth was monitored using ultrasound imaging. Once average tumor size reached 500 mm3, mice were treated twice a week with an i.v. injection of vehicle or PEGPH20 (4,500 μg/kg) (a–c). Statistical difference was tested using repeated measured two-way ANOVA and Bonferroni’s post hoc test.
