Accumulation of Extracellular Hyaluronan by Hyaluronan Synthase 3 Promotes Tumor Growth and Modulates the Pancreatic Cancer Microenvironment
Figure 5
Removal of extracellular hyaluronan decreases HIF-1α and Snail signaling. Vehicle- and PEGPH20- (2 doses; 4,500 μg/kg) treated BxPC-3 and BxPC-3 HAS3 tumors were homogenized and nuclear extracts were analyzed with Western blot using HIF-1α (a) and (b), Snail (d) and (e), β-actin (a) and (b), and Histone 3 (c) and (d) antibodies. In (a) and (b), the blot is a representative of three experiments, and each lane represents a piece of the same vehicle-treated or PEGPH20-treated tumor. The HIF-1α band is indicated with an arrow. In (d) and (e), lysates from the same vehicle-treated or PEGPH20-treated tumor were combined and each lane represents Snail level of one tumor. Intensities of the bands in the cropped blots were quantified using Image-Pro Analyzer 7.0 software and normalized to the intensity of the housekeeping protein ((c) and (f)). Data were plotted as mean ± S.D., and statistical difference between the groups was tested with test (; ; and ).
