Accumulation of Extracellular Hyaluronan by Hyaluronan Synthase 3 Promotes Tumor Growth and Modulates the Pancreatic Cancer Microenvironment

<table>Effect of HAS2 and HAS3 overexpression and hyaluronan removal on apoptosis and proliferation in BxPC-3, BxPC-3 HAS2, and BxPC-3 HAS3 tumors. Vehicle-treated and PEGPH20-treated (2 doses; 4,500 <i>μ</i>g/kg; <svg height="11.125" id="M23" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 36.237499 11.125" width="36.237499" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>/group) BxPC-3, BxPC-3 HAS2, and BxPC-3 HAS3 xenograft tumor sections were stained for CC3 and PH3 to visualize apoptotic and proliferative cells, respectively. Number of positive cells per tumor section was analyzed using Aperio Positive Pixel v9 and Nuclear staining algorithms, respectively, ((a) and (b)). Data were plotted as mean ± S.D., and statistical difference between the groups was tested with two-way ANOVA and Bonferroni’s post hoc test (<svg height="13.95" id="M24" style="vertical-align:-0.30096pt" version="1.1" viewbox="0 0 73.300003 13.95" width="73.300003" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>).</table>

BioMed Research International

fig6

Figure 6

Figure 6: Accumulation of Extracellular Hyaluronan by Hyaluronan Synthase 3 Promotes Tumor Growth and Modulates the Pancreatic Cancer Microenvironment 

Figure 6 | Accumulation of Extracellular Hyaluronan by Hyaluronan Synthase 3 Promotes Tumor Growth and Modulates the Pancreatic Cancer Microenvironment 