Research Article

Effect of High Hydrostatic Pressure Processing on Microbiological Shelf-Life and Quality of Fruits Pretreated with Ascorbic Acid or SnCl2

Table 3

Evolution of microbiological counts ( CFU/g), firmness, values, and sensory evaluation in HHP-treated at 600 MPa for 5 min at 10°C and untreated (control case) pear pieces in sucrose solution with or without ascorbic acid during storage (5°C). Each data point is an average ± standard deviation from three individual batches.

Time (days)Time (days)
067122185067122185

P. polymyxa Firmness
Control1.2 ± 0.22.4 ± 0.24.7 ± 0.5NDControl141.0 ± 12.570.5 ± 5.067.0 ± 8.057.0 ± 6.5
1st handling without ascorbic acid No growth detected (<dl during 185 days)1st handling without ascorbic acid 75.5 ± 7.5101.0 ± 8.096.0 ± 6.592.5 ± 9.5
1st handling with ascorbic acid No growth detected (<dl during 185 days)1st handling with ascorbic acid 75.5 ± 7.573.5 ± 5.578.5 ± 7.579.5 ± 7.0
3rd handling without ascorbic acid No growth detected (<dl during 185 days)3rd handling without ascorbic acid 100.0 ± 10.5116.5 ± 6.0122.5 ± 10.5116.5 ± 11.5
3rd handling with ascorbic acid No growth detected (<dl during 185 days)3rd handling with ascorbic acid 100.0 ± 10.5107.0 ± 9.5116.5 ± 11.0133.0 ± 12.5

Total Viable Counts value
Control1.4 ± 0.24.3 ± 0.46.9 ± 0.7NDControl39.5 ± 3.340.1 ± 4.038.6 ± 3.039.6 ± 2.4
1st handling without ascorbic acid No growth detected (<dl during 185 days)1st handling without ascorbic acid 38.8 ± 2.438.9 ± 2.640.3 ± 2.538.4 ± 1.0
1st handling with ascorbic acid No growth detected (<dl during 185 days)1st handling with ascorbic acid 37.4 ± 2.537.1 ± 3.140.0 ± 4.234.8 ± 3.7
3rd handling without ascorbic acid No growth detected (<dl during 185 days)3rd handling without ascorbic acid 36.9 ± 3.136.1 ± 2.437.8 ± 1.737.4 ± 2.2
3rd handling with ascorbic acid No growth detected (<dl during 185 days)3rd handling with ascorbic acid 37.1 ± 1.636.9 ± 2.034.7 ± 3.231.4 ± 2.8

Lactic acid bacteria
Control<dl1.2 ± 0.12.0 ± 0.4NDControl0.01.22.0ND
1st handling without ascorbic acid No growth detected (<dl during 185 days)1st handling without ascorbic acid 0.00.40.50.5
1st handling with ascorbic acid No growth detected (<dl during 185 days)1st handling with ascorbic acid 0.10.30.30.8
3rd handling without ascorbic acid No growth detected (<dl during 185 days)3rd handling without ascorbic acid 0.11.41.41.4
3rd handling with ascorbic acid No growth detected (<dl during 185 days)3rd handling with ascorbic acid 0.00.80.60.8

Yeasts
Control<dl4.1 ± 0.56.3 ± 0.6NDControl10.06.43.1ND
1st handling without ascorbic acid No growth detected (<dl during 185 days)1st handling without ascorbic acid 10.08.04.65.6
1st handling with ascorbic acid No growth detected (<dl during 185 days)1st handling with ascorbic acid 10.08.58.37.9
3rd handling without ascorbic acid No growth detected (<dl during 185 days)3rd handling without ascorbic acid 10.03.04.03.5
3rd handling with ascorbic acid No growth detected (<dl during 185 days)3rd handling with ascorbic acid 10.05.55.95.8

ND: not determined-spoiled product, dl: detection limit (1  CFU/g); athe taste is the mean value of scores given by 8 panelists-0: good, 1: acceptable, 2: spoiled; bthe total evaluation is the mean value of odor, color, texture, and taste of 8 panelists. -10: very good, 5: acceptable, and 1: totally unacceptable.