Hepatitis B Virus X Upregulates HuR Protein Level to Stabilize HER2 Expression in Hepatocellular Carcinoma Cells
Figure 2
The HER2 mRNA expression was stabilized in HBx-expressing HCC cells. (a) The two HBx-paired HCC cells were treated with proteasomal inhibitors (MG132 and bortezomib) for 24 hrs. The HER2 protein expression was analyzed by Western blot (). (b) The HER2 mRNA expression in two HBx-paired HCC cells was examined by RT-qPCR. The HER2 mRNA expression was normalized to actin expression. Statistical analysis was performed by Student’s -test. ; as compared to each control group (). (c) The HepG2 and HepG2x HCC cells were treated with 5 μM Actinomycin D for indicated time periods. The relative remaining HER2 mRNA expression in each of the HCC cells was determined by RT-qPCR. The HER2 mRNA expression was normalized to actin expression (). (d) Hep3B HCC cells were transiently transfected with myc-HBx expression vector for 48 hrs, followed by treatment of 5 μM Actinomycin D. The relative remaining HER2 mRNA expression in each group was determined by RT-qPCR. The HER2 mRNA expression was normalized to actin expression. The protein expression of myc-HBx was confirmed by Western blot. Statistical analysis was performed by Student’s -test. as compared to each control group. N.S. denoted “not significant” ().
