Hepatitis B Virus X Upregulates HuR Protein Level to Stabilize HER2 Expression in Hepatocellular Carcinoma Cells
Figure 3
HBx increased HER2 protein expression by HuR-dependent mRNA stabilization in HCC cells. (a) The HuR protein expression in HBx-paired HCC cells was examined by Western blot (). (b) Hep3Bx cells were transiently transfected with either si-control or si-HuR for 4 days. The protein expressions of HER2, EGFR, and HuR were analyzed by Western blot (). (c) Hep3Bx cells were transiently transfected with either si-control or si-HuR for 4 days, followed by the treatment of 5 μM Actinomycin D. The relative remaining HER2 mRNA expression in each group was determined by RT-qPCR. The HER2 mRNA expression was normalized to actin expression. Statistical analysis was performed by Student’s -test. ; as compared to each control group (). (d) Transient transfection of HBx siRNA was performed in Hep3B cells for 48 hrs, followed by overexpression of myc-HBx expression vector for another 48 hrs. Whole cell lysates were harvested for the examination of HuR and myc-HBx protein expressions by Western blot ().
