Repeated Batch Fermentation Biotechnology for the Biosynthesis of Lipid and Gamma-Linolenic Acid by Cunninghamella bainieri 2A1
Table 2
Central composite design and experimental results for the biomass concentration, lipid concentration, lipid content, and GLA concentration produced by Cunninghamella bainieri 2A1 in repeated batch fermentation at 30°C for 96 h.
Run
C/N
Biomass (g/L)
Lipid concentration (g/L)
Lipid content (%)
GLA (g/L)
1
0
0
+1
29.45
21.5
6.1
28.3
0.33
2
+1
−1
−1
71.5
17.65
2.85
16.1
0.17
3
+1
−1
+1
71.5
15.95
5.35
33.5
0.15
4
0
0
0
29.45
20.9
4.5
21.5
0.21
5
0
+1
0
20.38
19.6
4.1
20.9
0.22
6
−1
−1
−1
35
17.15
3.0
17.4
0.45
7
+1
+1
−1
27.5
20.4
3.26
15.9
0.40
8
−1
0
0
19.45
19.5
4.4
22.5
0.18
9
0
0
−1
29.45
20.8
3.2
15.3
0.30
10
0
0
0
29.45
21.0
4.5
21.4
0.19
11
0
0
0
29.45
21.5
4.7
21.8
0.15
12
−1
−1
+1
35
16.65
5.95
35.7
0.40
13
0
0
0
25.1
21.15
4.5
21.2
0.18
14
+1
+1
+1
27.5
21.1
5.3
25.1
0.28
15
−1
+1
−1
13.46
19.2
2.4
14.5
0.35
16
0
−1
0
53
16.3
4.3
26.3
0.12
17
+1
0
0
39.73
19.7
4.35
22.0
0.20
18
−1
+1
+1
13.46
20.4
4.85
24.0
0.29
: glucose concentration (g/L); : ammonium tartrate (g/L); : harvesting time (h); C/N: the ratio of initial carbon content (mol) to nitrogen content (mol) used in medium.
