Repeated Batch Fermentation Biotechnology for the Biosynthesis of Lipid and Gamma-Linolenic Acid by Cunninghamella bainieri 2A1

Table 3

Analysis of variance for the second-order polynomial model of biomass synthesis by Cunninghamella bainieri 2A1 using repeated batch cultivation at 30°C for 96 h.

Source	Polynomial coefficients	Sum of squares	DF	Mean square	𝐹 value	Prob > 𝐹
Model		58.56	9	6.51	22.88	<0.0001**
Intercept	20.73
𝑋1	0.19	0.36	1	0.36	1.27	0.2926
𝑋2	1.70	28.90	1	28.90	101.61	<0.0001**
𝑋3	0.040	0.016	1	0.016	0.056	0.8185
𝑋^21	−0.72	1.42	1	1.42	4.98	0.0561
𝑋^22	−2.37	15.26	1	15.26	53.66	<0.0001**
𝑋^23	0.83	1.85	1	1.85	6.51	0.0341*
𝑋1𝑋2	0.26	0.55	1	0.55	1.94	0.2014
𝑋1𝑋3	−0.21	0.36	1	0.36	1.27	0.2924
𝑋2𝑋3	0.51	2.10	1	2.10	7.39	0.0263*
Residuals		2.28	8	0.28
Lack of fit		2.07	5	0.41	6.0	0.0856
Pure error		0.21	3	0.069

∗Statistically significant at 95% probability level.
∗∗Statistically significant at 99% of probability level.
𝑋₁: glucose concentration (g/L); 𝑋₂: ammonium tartrate (g/L); 𝑋₃: harvesting time (h). 𝑋²₁, 𝑋²₂, and 𝑋²₃: the quadratic terms; 𝑋₁𝑋₂,  𝑋₁𝑋₃, and 𝑋₂𝑋₃: the interaction terms.
𝑅²=0.9626.