Repeated Batch Fermentation Biotechnology for the Biosynthesis of Lipid and Gamma-Linolenic Acid by Cunninghamella bainieri 2A1

Table 7

Fatty acid compositions of cellular lipid produced by Cuninghamella bainieri 2A1 in different repeated batch culture runs according to CCD.

Run	𝑋1	𝑋2	𝑋3	^Δ6,9,12C18:3	^Δ9,12C18:2	^Δ9C18:1	C18:0	C16:0
1	0	0	+1	0.33	0.98	2.04	1.26	1.06
2	+1	−1	−1	0.17	0.31	0.9	0.37	0.38
3	+1	−1	+1	0.15	0.87	1.56	0.91	0.82
4	0	0	0	0.21	0.47	1.48	0.9	0.72
5	0	+1	0	0.22	0.59	1.53	0.93	0.72
6	−1	−1	−1	0.45	0.32	0.94	0.38	0.41
7	+1	+1	−1	0.40	0.38	1.21	0.7	1.63
8	−1	0	0	0.18	0.16	1.77	0.87	0.8
9	0	0	−1	0.30	0.25	0.76	0.32	0.36
10	0	0	0	0.19	0.50	1.38	0.90	0.70
11	0	0	0	0.15	0.46	0.9	0.98	0.78
12	−1	−1	+1	0.40	0.78	2.31	1.0	1.04
13	0	0	0	0.18	0.55	1.3	0.88	0.6
14	+1	+1	+1	0.28	0.92	1.69	1.06	0.93
15	−1	+1	−1	0.35	0.28	0.92	0.34	0.39
16	0	−1	0	0.12	0.41	1.62	1.04	0.83
17	+1	0	0	0.20	0.54	1.51	0.87	0.7
18	−1	+1	+1	0.29	0.11	2.36	1.13	1.17

𝑋₁: glucose (g/L); 𝑋₂: ammonium tartrate (g/L); 𝑋₃: harvesting time (h); ^Δ6,9,12C18:3: gamma-linolenic acid (g/L); ^Δ9,12C18:2: linoleic acid (g/L); ^Δ9C18:1: oleic acid (g/L); C18:0: stearic acid (g/L); C16:0: palmitic acid (g/L).