Growth Inhibition by Bupivacaine Is Associated with Inactivation of Ribosomal Protein S6 Kinase 1
Figure 1
Dose-dependent inhibition of cell growth. (a) NIH-3T3, C2C12, and C6 neuroglial cells were plated at a cell density of 5 103 cells /well in triplicate under standard growth conditions (96-well format) for 24 hrs and incubated with indicated concentrations of bupivacaine. Cells were washed with cold PBS and subjected to MTT assays. (b) Dose-dependent inhibition of cell growth in NIH-3T3 cells exposed to bupivacaine at indicated concentrations for 24 hrs. (c) Time dependent inhibition of cell growth in NIH cells treated with bupivacaine (0.614 mM). Cell growth rate was normalized to the control. Data are mean ± SEM (). versus control.
