Growth Inhibition by Bupivacaine Is Associated with Inactivation of Ribosomal Protein S6 Kinase 1
Figure 5
Overlapping time course of inhibition between Erk and S6K1 indicates bupivacaine as specific inhibitor of Erk and S6K1: (a) NIH-3T3 cells treated with indicated concentrations of bupivacaine as above. Lysates were clarified, immunoprecipitated, and probed with indicated antibodies. (b) Densitometric analysis of anti-P-Erk1 signals (normalized to total Erk levels) from three independent experiments. Data are relative Erk activation levels with control set as 100%, presented as mean ± SEM.
