CD163+ Tumor-Associated Macrophages Correlated with Poor Prognosis and Cancer Stem Cells in Oral Squamous Cell Carcinoma

Figure 1

Human OSCC tissue array analysis revealed that CD68 and CD163 were overexpressed in human OSCCs. (a) Representative HE staining and immunohistochemical staining (IHC) of CD68 and CD163 in human oral cancer tissue (right) compared with normal oral mucosa (left) (scale bars = 100 𝜇mol). (b) Quantitative histoscore of CD68 and CD163 expression in normal oral mucosa, oral epithelial dysplasia, and human oral cancer; CD68 levels were significantly higher when compared with normal oral mucosa (mean ± SEM; ^∗,𝑃<0.05; ^∗∗,𝑃<0.01). (c) The expressions of CD68 and CD163 were correlated with lymph node status of human oral cancer (quantification was done using Aperio nuclear quantification software, and statistics was calculated using Graph Pad Prism 5. Mean ± SEM; ^∗𝑃<0.05; ^∗∗𝑃<0.01). (d) Overall survival of the OSCC patients with CD68 and CD163 expression calculated and presented by Kaplan-Meier analysis, and CD163 expression was significantly correlated with overall survival (𝑃=0.0319, 𝑛=38).