Impact Assessment of Cadmium Toxicity and Its Bioavailability in Human Cell Lines (Caco-2 and HL-7702)

Table 1

Activities of antioxidants enzymes (SOD, GPX) and differentiation marker enzyme (AKP) in Caco-2 cells and HL-7702 cells at different Cd levels (after 2 hr exposure).

Parameters	Cell lines	Cd concentration (mg L^−1)
		0	0.25	0.5	2	4	8	10
GPx (U mg^−1 cell protein)	Caco-2 cells	198.7±0.7^a	190.9±2.6^b	183.9±3.2^c	133.7±0.8^d	70.1±1.1^e	48.09±0.9^f	30.3±0.7^g
	HL-7702 cells	306.9±2.3^a	300.2±8.7^ab	295.6±5.3^b	207.2±1.1^c	144.9±0.8^d	100.3±0.6^e	60.4±0.6^f
SOD (U mg^−1 cell protein)	Caco-2 cells	26.2±1.8^a	25.1±3^a	22.91±2.4^ab	21.8±0.5^bc	21.7±0.9^bc	19.0±2^cd	15.9±0.3^d
	HL-7702 cells	26.0±1.8^a	24.5±0.6^a	24.0±0.2^a	21.4±1.0^b	20.03±2.0^b	15.2±0.5^c	11.1±1.1^d
AKP (%Ck)	Caco-2 cells	100±0.1^a	97.6±3.3^b	95.3±3.6^b	83.1±0.7^c	79.41±0.7^d	75.0±1.0^e	66.1±0.6^f
	HL-7702 cells	100±0.4^a	95.5±5.1^b	90.7±3.7^c	65.32±0.9^d	45.97±1.0^e	28.31±1.3^f	27.01±1.0^f

Multiple comparison tests were for Caco-2 cells and HL-7702 cells at different Cd levels and different letters indicate significant differences at 𝑃<0.05 as calculated by the least significant difference (LSD) test. Values are presented as mean ± S.D.; 𝑛=3.