Construction and Characterization of a Bacterial Artificial Chromosome Library for the Hexaploid Wheat Line 92R137
Figure 2
Size selections of the partially digested products of genomic DNA of wheat line 92R137. (a) The first size selection of the partially digested products of genomic DNA. Plug pieces were separated on 1% agarose gel in 0.5x TBE buffer and run in a CHEF DR-III System (Bio-Rad) at 6 V/cm, 1–50 s switch time, linear ramp, 120° angle at 14°C for 18 h. (b) The second size selection of the partially digested products of genomic DNA. The first size selected fractions were separated on 1% agarose gel in 0.5x TBE buffer and run at 6 V/cm 3–5 s switch time, linear ramp, 120° angle at 14°C for 18 h. M: the λ PFG marker.
