Development and Retranslational Validation of an In Vitro Model to Characterize Acute Infections in Large Human Joints

Figure 4

Detecting aggrecan in in vivo and in vitro samples. Significantly increased aggrecan (ACAN) levels were detected by ELISA in lavage fluids from acutely infected knee joints ((a) **). This was likewise observed in cocultures after prolonged LPS PMA challenge ((b) day 10 *). LPS and LPS PMA challenge results in slightly increased ACAN mRNA expression in CHDR ((c) days 3–10) and SFB ((d) day 3). Statistics: (a) Mann-Whitney test and (b)–(d) Student’s t-test, ^∗𝑃<0.05 and ^∗∗𝑃<0.01. ELISA data were normalized versus total protein content (TP).

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