Development and Retranslational Validation of an In Vitro Model to Characterize Acute Infections in Large Human Joints

Figure 5

Detecting BMP-7 in in vivo and in vitro samples. Significantly elevated BMP-7 levels were detected by ELISA in lavage fluids from acutely infected knee joints ((a) **) also observed in cocultures after prolonged challenge with LPS PMA ((b) day 10 **). While no BMP-7 mRNA expression in CHDR was detected ((c) n.d.), a significant increase in BMP-7 mRNA expression was observed in SFB after either an LPS ((c) day 7 *) or LPS PMA challenge ((c) day 10 *). Statistics: (a) Mann-Whitney test and (b), (c) Student’s t-test, ^∗𝑃<0.05 and ^∗∗𝑃<0.01. ELISA data were normalized versus total protein content (TP).

(a)  

(b)

(c)