Development and Retranslational Validation of an In Vitro Model to Characterize Acute Infections in Large Human Joints

Figure 7

Synovial fibroblasts (SFB) proliferation. Originally in all cocultures, 2∗10⁵ SFB were seeded per well. While the SFB numbers increased steadily in controls, cell numbers in LPS decreased significantly (day 10, ^∗∗𝑃<0.01) and LPS PMA assays (day 7, ^∗𝑃<0.05; day 10, ^∗∗𝑃<0.01). This observation is supported by giemsa-stained SFB on day 10 (b, left: control; middle: LPS; right: LPS PMA challenged cocultures). Statistics: (a) Student’s t-test, ^∗𝑃<0.05, ^∗∗𝑃<0.01; cell number was determined by ImageJ. Scale bar 100 μm.

(a)

(b)