Atg6/UVRAG/Vps34-Containing Lipid Kinase Complex Is Required for Receptor Downregulation through Endolysosomal Degradation and Epithelial Polarity during Drosophila Wing Development

Figure 11

RNAi of UVRAG but Atg14 alters cell polarity and membrane protein localization. Semiconfocal immunodetection showing XY (marked by a letter p (plan view) at the bottom left corners) and XZ sections (marked by a letter s (side view) at the bottom left corners) in the plane of pupal wings at 32 h APF. Regions of controls are shown in boxes marked by a single character at the upper left side, whereas the regions where dsRNA for Atg6 was expressed by en-Gal4 driver are shown in boxes marked by a character at the upper left side with an apostrophe. (a), (c), (e), and (g) Immunodetection reveals no obvious differences of the pattern of wing hairs and the localization of Fas III, Arm, and β-int between control and Atg14 depleted regions of pupal wings. (b), (d), (f), and (h) In contrast to Atg14, the RNAi of UVRAG dramatically alters wing hair development and the localization of membrane proteins (Fas III, Arm, and β-int) and these phenotypes are very similar to those induced by Atg6 RNAi as was shown in Figure 2. For genotypes, see Table S4. Scale bars represent 5 μm.