Research Article

Atg6/UVRAG/Vps34-Containing Lipid Kinase Complex Is Required for Receptor Downregulation through Endolysosomal Degradation and Epithelial Polarity during Drosophila Wing Development

Figure 7

Enhanced Notch signaling in Atg6 RNAi and UVRAG RNAi cells. Larval wing discs expressing RNAi with or without transgenic Notch. UAS-myrRFP was used to mark enGal4-specific region. Notch response element- (NRE-) EGFP was used to show Notch transcriptional activity. (a) Atg6 RNAi and (b) Notch overexpression lead to enhanced Notch activity. (c) This effect was further increased as coexpression of Atg6 RNAi and Notch strongly enhanced the reporter expression. (d) UVRAG enhanced Notch transcriptional activity similarly to Atg6 RNAi (e) RNAi of Atg14 did not alter the reporter expression. (f) Control disc. Histograms show the intensity profiles of the NRE-GFP expression in the enGal4-specific regions. Note that the peaks are broader in panels (a)–(d), compared to panels (e), (f). For genotypes, see Table S4. Scale bars represent 50 μm.
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